When eaten, similar to a Glow Berry, it will allow the player to glow in the dark. When used as fuel for Moggles it will only restore 6.25% durability.
A Lesser Glow Berry will also cause any creature that consumes it, including Bunnymen, Pigs, Splumonkeys, Prime Apes and Gobblers, to glow. The intensity of the glowing light decreases over time and will extinguish 60 seconds after consumption. If dropped on the ground, it will glow, similar to a Light Bulb. It emits enough light to prevent an attack from Charlie.